Induction of apoptosis via proteasome inhibition in leukemia/lymphoma cells by two potent piperidones.

ABSTRACT:

Purpose

Previously, compounds containing a piperidone structure have been shown to be highly cytotoxic to cancer cells. Recently, we found that the piperidone compound P2 exhibits a potent anti-neoplastic activity against human breast cancer-derived cells. Here, we aimed to evaluate two piperidone compounds, P1 and P2, for their potential anti-neoplastic activity against human leukemia/lymphoma-derived cells.

Methods

Cytotoxicity and apoptosis induction were evaluated using MTS, annexin V-FITC/PI and mitochondrial membrane potential polychromatic assays to confirm the mode of action of the piperidone compounds. The effects of compound P1 and P2 treatment on gene expression were assessed using AmpliSeq analysis and, subsequently, confirmed by RT-qPCR and Western blotting.

SUBMITTER: Contreras L 

PROVIDER: S-EPMC6241245 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature