Uncontrolled Diabetes Mellitus Has No Major Influence on the Platelet Transcriptome.

ABSTRACT:

Background

Diabetes mellitus (DM) has been associated with increased platelet reactivity as well as increased levels of platelet RNAs in plasma. Here, we sought to evaluate whether the platelet transcriptome is altered in the presence of uncontrolled DM.

Methods

Next-generation sequencing (NGS) was performed on platelet RNA for 5 patients with uncontrolled DM (HbA1c 9.0%) and 5 control patients (HbA1c 5.5%) with otherwise similar clinical characteristics. RNA was isolated from leucocyte-depleted platelet-rich plasma. Libraries of platelet RNAs were created separately for long RNAs after ribosomal depletion and for small RNAs from total RNA, followed by next-generation sequencing.

Results

Platelets in both groups demonstrated RNA expression profiles characterized by ab

SUBMITTER: Nuhrenberg TG 

PROVIDER: S-EPMC6241365 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature