Post-Translational Tyrosine Geranylation in Cyanobactin Biosynthesis.

ABSTRACT: Prenylation is a widespread modification that improves the biological activities of secondary metabolites. This reaction also represents a key modification step in biosyntheses of cyanobactins, a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) produced by cyanobacteria. In cyanobactins, amino acids are commonly isoprenylated by ABBA prenyltransferases that use C₅ donors. Notably, mass spectral analysis of piricyclamides from a fresh-water cyanobacterium suggested that they may instead have a C₁₀ geranyl group. Here we characterize a novel geranyltransferase involved in piricyclamide biosynthesis. Using the purified enzyme, we show that the enzyme PirF catalyzes Tyr O-geranylation, which is an unprecedented post-translational modifi

SUBMITTER: Morita M 

PROVIDER: S-EPMC6242345 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature