Convective forces increase CXCR4-dependent glioblastoma cell invasion in GL261 murine model.

ABSTRACT: Glioblastoma is the most common and malignant form of brain cancer. Its invasive nature limits treatment efficacy and promotes inevitable recurrence. Previous in vitro studies showed that interstitial fluid flow, a factor characteristically increased in cancer, increases glioma cell invasion through CXCR4-CXCL12 signaling. It is currently unknown if these effects translate in vivo. We used the therapeutic technique of convection enhanced delivery (CED) to test if convective flow alters glioma invasion in a syngeneic GL261 mouse model of glioblastoma. The GL261 cell line was flow responsive in vitro, dependent upon CXCR4 and CXCL12. Additionally, transplanting GL261 intracranially increased the populations of CXCR4⁺ and double positive cells versus 3D culture. We showed that indu

SUBMITTER: Cornelison RC 

PROVIDER: S-EPMC6242861 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature