Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells.

ABSTRACT: Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-βH1 and EndoC-βH2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca²⁺]_i, membrane depolarisation and increased action potential firing. Similar to human primary beta cells, K_ATP channel activity is low at 1 mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the K_ATP

SUBMITTER: Hastoy B 

PROVIDER: S-EPMC6242937 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature