Single cell RNA-seq and ATAC-seq analysis of cardiac progenitor cell transition states and lineage settlement.

ABSTRACT: Formation and segregation of cell lineages forming the heart have been studied extensively but the underlying gene regulatory networks and epigenetic changes driving cell fate transitions during early cardiogenesis are still only partially understood. Here, we comprehensively characterize mouse cardiac progenitor cells (CPCs) marked by Nkx2-5 and Isl1 expression from E7.5 to E9.5 using single-cell RNA sequencing and transposase-accessible chromatin profiling (ATAC-seq). By leveraging on cell-to-cell transcriptome and chromatin accessibility heterogeneity, we identify different previously unknown cardiac subpopulations. Reconstruction of developmental trajectories reveal that multipotent Isl1⁺ CPC pass through an attractor state before separating into different developmental bran

SUBMITTER: Jia G 

PROVIDER: S-EPMC6242939 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature