Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures.

ABSTRACT: Osteoarthritis (OA) is one of the most common causes of disability and represents a major socio-economic burden. Despite intensive research, the molecular mechanisms responsible for the initiation and progression of OA remain inconclusive. In recent years experimental findings revealed elevated levels of reactive oxygen species (ROS) as a major factor contributing to the onset and progression of OA. Hence, we designed a hydrostatic pressure bioreactor system that is capable of stimulating cartilage cell cultures with elevated ROS levels. Increased ROS levels in the media did not only lead to an inhibition of glycosaminoglycans and collagen II formation but also to a reduction of already formed glycosaminoglycans and collagen II in chondrogenic mesenchymal stem cell pellet cultures. These e

SUBMITTER: Rieder B 

PROVIDER: S-EPMC6242959 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature