Genetic Identification of Echinococcus granulosus Isolates in Hamadan, Western Iran.

ABSTRACT:

Background

Cystic echinococcosis is a zoonotic infection and considered as a major economic and public health concern worldwide. This research was conducted to determine genotypic characteristics of livestock and human hydatid cyst isolates from Hamadan area, western Iran.

Methods

Sampling was conducted in Hamadan industrial slaughterhouse and Beast Hospital of Hamadan City, western Iran, from 2015 to 2016. Overall, 74 livestock isolates including 69 sheep, 3 cattle and 2 goats and 9 human hydatid cysts were genotyped by PCR amplification of the rDNA ITS1 region and followed restriction fragment length polymorphism (RFLP) analysis with four restriction endonuclease enzymes, RsaI, HpaII, AluI, and TaqI, and sequencing.

Results

The PCR amplic

SUBMITTER: Matini M 

PROVIDER: S-EPMC6243157 | biostudies-literature | 2018 Jul-Sep

REPOSITORIES: biostudies-literature