Cell engineering with microfluidic squeezing preserves functionality of primary immune cells in vivo.

ABSTRACT: The translational potential of cell-based therapies is often limited by complications related to effectively engineering and manufacturing functional cells. While the use of electroporation is widespread, the impact of electroporation on cell state and function has yet to be fully characterized. Here, we use a genome-wide approach to study optimized electroporation treatment and identify striking disruptions in the expression profiles of key functional transcripts of human T cells. These genetic disruptions result in concomitant perturbation of cytokine secretion including a 648-fold increase in IL-2 secretion (P < 0.01) and a 30-fold increase in IFN-γ secretion (P < 0.05). Ultimately, the effects at the transcript and protein level resulted in functional deficiencies in vivo

SUBMITTER: DiTommaso T 

PROVIDER: S-EPMC6243275 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature