Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism.

ABSTRACT: [FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H₂-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

SUBMITTER: Wegelius A 

PROVIDER: S-EPMC6243476 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature