Genetic Factors Involved in the Development and Progression of Nonalcoholic Fatty Liver Disease.

ABSTRACT:

Purpose

The aim of our study was to identify the possible involvement of adiponutrine polymorphysm and of human leukocyte antigens (HLA) in the development of non-alcoholic fatty liver disease (NAFLD).

Material and methods

We included in this study a total of 138 subjects with non-invasive diagnosis of non-alcoholic hepatic steatosis. The patatin-like phospholipase domain containing protein 3 (PNPLA3) rs738409 (adiponutrine) polymorphism was genotyped by allelic discrimination TaqMan PCR assay (5' nuclease assay), using predesigned TaqMan SNP Genotyping Assays. Class I and II HLA antigens were determined by the polymerase chain reaction sequence specific oligonucleotide method (ADN-PCR-SSO). The results were compared with the same data from the control group subjects.

Res

SUBMITTER: Amzolini AM 

PROVIDER: S-EPMC6243520 | biostudies-literature | 2015 Oct-Dec

REPOSITORIES: biostudies-literature