Wash-free, label-free immunoassay for rapid electrochemical detection of PfHRP2 in whole blood samples.

ABSTRACT: Currently, the diagnosis of many diseases relies on laboratory-based immunoassays (ELISA, Western Blot), which are laborious, time-consuming and expensive. To address these limitations, we report a wash-free and label-free electrochemical immunoassay for rapid measurements of protein biomarkers in blood samples. This immunosensor employs a unique detection scheme based on electrochemical-chemical (EC) redox cycling for signal amplification combined with an affinity-based protein quantification strategy. All of the reagents required for this assay are dried and stored on a stacked membrane assembly, consisting of a Vivid Plasma Separation membrane and two cellulose membranes situated above the sensor, enabling excellent stability at room temperature for up to 2 months. Proof of concept was

SUBMITTER: Dutta G 

PROVIDER: S-EPMC6244414 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature