Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus.

ABSTRACT: Protein transfection is a versatile tool to study or manipulate cellular processes and also shows great therapeutic potential. However, the repertoire of cost effective techniques for efficient and minimally cytotoxic delivery remains limited. Mesoporous silica nanoparticles (MSNs) are multifunctional nanocarriers for cellular delivery of a wide range of molecules, they are simple and economical to synthesize and have shown great promise for protein delivery. In this work we present a general strategy to optimize the delivery of active protein to the nucleus. We generated a bimolecular Venus based optical sensor that exclusively detects active and bioavailable protein for the performance of multi-parameter optimization of protein delivery. In conjunction with cell viability tests we maximi

SUBMITTER: Chiu HY 

PROVIDER: S-EPMC6244737 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature