Development and evaluation of gene expression biomarkers for chemical pollution in common frog (Rana temporaria) tadpoles.

ABSTRACT: Pollutants have been proposed as one factor in the worldwide declines of amphibian species and populations. Applying gene expression analysis of liver RNA in tadpoles would be a possible approach for biomarker measurements to increase knowledge of ecological health in amphibian populations. The major aim of this study was to explore the relevance of applying gene expression analyses of cytochrome p450 (cyp1a), metallothionein (mt), and vitellogenin (vtg) in Rana temporaria tadpoles. Therefore, tadpoles were exposed for 1 week to β-naphthoflavone (BNF), cadmium chloride (CdCl₂), and ethinylestradiol (EE2). Primers were developed for RT-qPCR to analyze gene expression in livers. The result showed that the methods for gene expression analyses of cyp1a, mt, and vtg as well as the re

SUBMITTER: Carlsson G 

PROVIDER: S-EPMC6245027 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature