Effect of Chronic Hyperglycemia on Glucose Metabolism in Subjects With Normal Glucose Tolerance.

ABSTRACT: Chronic hyperglycemia causes insulin resistance, but the inheritability of glucotoxicity and the underlying mechanisms are unclear. We examined the effect of 3 days of hyperglycemia on glucose disposal, enzyme activities, insulin signaling, and protein O-GlcNAcylation in skeletal muscle of individuals without (FH^-) or with (FH⁺) family history of type 2 diabetes. Twenty-five subjects with normal glucose tolerance received a [3-³H]glucose euglycemic insulin clamp, indirect calorimetry, and vastus-lateralis biopsies before and after 3 days of saline (n = 5) or glucose (n = 10 FH^- and 10 FH⁺) infusion to raise plasma glucose by ∼45 mg/dL. At baseline, FH⁺ had lower insulin-stimulated glucose oxidation and t

SUBMITTER: Shannon C 

PROVIDER: S-EPMC6245228 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature