TransFlow: a modular framework for assembling and assessing accurate de novo transcriptomes in non-model organisms.

ABSTRACT:

Background

The advances in high-throughput sequencing technologies are allowing more and more de novo assembling of transcriptomes from many new organisms. Some degree of automation and evaluation is required to warrant reproducibility, repetitivity and the selection of the best possible transcriptome. Workflows and pipelines are becoming an absolute requirement for such a purpose, but the issue of assembling evaluation for de novo transcriptomes in organisms lacking a sequenced genome remains unsolved. An automated, reproducible and flexible framework called TransFlow to accomplish this task is described.

Results

TransFlow with its five independent modules was designed to build different workflows depending on the nature of the original reads. This architecture enables diff

SUBMITTER: Seoane P 

PROVIDER: S-EPMC6245506 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature