Proteinase K treatment improves RNA recovery from thyroid cells fixed with liquid-based cytology solution.

ABSTRACT:

Objective

Fine-needle aspiration biopsy (FNAB), an important diagnostic tool given its simplicity, safety, and cost-effectiveness, is fast becoming a popular procedure in the diagnosis of thyroid diseases. Generally, cells isolated from biopsies are transferred directly to microscope slides to prepare smears for cytopathological examination; however, the technical difficulties of this procedure often cause poor reproducibility, which limits the accuracy of diagnostic results. Liquid-based cytology (LBC), in which isolated cells are collected in a fixative solution, is advantageous in that it facilitates the preparation of homogenous cytological specimens. However, LBC has not been applied to molecular diagnoses, such as RNA expression-based diagnosis, mainly because of difficulties

SUBMITTER: Jikuzono T 

PROVIDER: S-EPMC6245812 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature