A highly multiplexed broad pathogen detection assay for infectious disease diagnostics.

ABSTRACT: Rapid pathogen identification during an acute febrile illness is a critical first step for providing appropriate clinical care and patient isolation. Primary screening using sensitive and specific assays, such as real-time PCR and ELISAs, can rapidly test for known circulating infectious diseases. If the initial testing is negative, potentially due to a lack of developed diagnostic assays or an incomplete understanding of the pathogens circulating within a geographic region, additional testing would be required including highly multiplexed assays and metagenomic next generation sequencing. To bridge the gap between rapid point of care diagnostics and sequencing, we developed a highly multiplexed assay designed to detect 164 different viruses, bacteria, and parasites using the NanoString nC

SUBMITTER: Koehler JW 

PROVIDER: S-EPMC6245831 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature