Automated sequential chromogenic IHC double staining with two HRP substrates.

ABSTRACT: Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.

SUBMITTER: Petersen KH 

PROVIDER: S-EPMC6245840 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature