Loss of DNA methylation is related to increased expression of miR-21 and miR-146b in papillary thyroid carcinoma.

ABSTRACT:

Background

DNA methylation in miRNA genes has been reported as a mechanism that may cause dysregulation of mature miRNAs and consequently impact the gene expression. This mechanism is largely unstudied in papillary thyroid carcinomas (PTC).

Methods

To identify differentially methylated miRNA-encoding genes, we performed global methylation analysis (Illumina 450 K), integrative analysis (TCGA database), data confirmation (pyrosequencing and RT-qPCR), and functional assays.

Results

Methylation analysis revealed 27 differentially methylated miRNA genes. The integrative analyses pointed out miR-21 and miR-146b as potentially regulated by methylation (hypomethylation and increased expression). DNA methylation and expression patterns of miR-21 and miR-146b were confirmed as

SUBMITTER: Ortiz IMDP 

PROVIDER: S-EPMC6245861 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature