Exploration of exosomal microRNA expression profiles in pigeon 'Milk' during the lactation period.

ABSTRACT:

Background

Pigeon crop has the unique ability to produce a nutrient rich substance termed pigeon 'milk' (PM), which has functional resemblance with the mammalian milk. Previous researches have demonstrated that a large number of exosomes and exosomal miRNAs exist in mammalian milk, and many of them are associated with immunity, growth and development. However, to date, little is known about the exosomes and exosomal miRNAs in PM.

Results

In this study, we isolated the exosomes from PM and used small RNA sequencing to investigate the distribution and expression profiles of exosomal miRNAs. A total of 301 mature miRNAs including 248 conserved and 53 novel miRNAs were identified in five lactation stages i.e. 1d, 5d, 10d, 15d, and 20d. From these, four top 10 conserved miRNAs (c

SUBMITTER: Ma Y 

PROVIDER: S-EPMC6245878 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature