Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing.

ABSTRACT:

Background

Antimicrobial peptides (AMPs) are multifunctional effector molecules that often combine direct antimicrobial activities with signaling or immunomodulatory functions. The skin secretions of anurans contain a variety of such bioactive peptides. The identification of AMPs from frog species often requires sacrificing several specimens to obtain small quantities of crude peptides, followed by activity based fractionation to identify the active principles.

Results

We report an efficient alternative approach to selectively amplify AMP-coding transcripts from very small amounts of tissue samples, based on RNA extraction and cDNA synthesis, followed by PCR amplification and high-throughput sequencing of size-selected amplicons. This protocol exploits the highly conserved s

SUBMITTER: Roncevic T 

PROVIDER: S-EPMC6245896 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature