Integrative analysis of fitness and metabolic effects of plasmids in Pseudomonas aeruginosa PAO1.

ABSTRACT: Horizontal gene transfer (HGT) mediated by the spread of plasmids fuels evolution in prokaryotes. Although plasmids provide bacteria with new adaptive genes, they also produce physiological alterations that often translate into a reduction in bacterial fitness. The fitness costs associated with plasmids represent an important limit to plasmid maintenance in bacterial communities, but their molecular origins remain largely unknown. In this work, we combine phenomics, transcriptomics and metabolomics to study the fitness effects produced by a collection of diverse plasmids in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Using this approach, we scan the physiological changes imposed by plasmids and test the generality of some main mechanisms that have been proposed to explain the c

SUBMITTER: San Millan A 

PROVIDER: S-EPMC6246594 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature