Major TCR Repertoire Perturbation by Immunodominant HLA-B^*44:03-Restricted CMV-Specific T Cells.

ABSTRACT: Lack of disease during chronic human cytomegalovirus (CMV) infection depends on the maintenance of a high-frequency CMV-specific T cell response. The composition of the T cell receptor (TCR) repertoire underlying this response remains poorly characterised, especially within African populations in which CMV is endemic from infancy. Here we focus on the immunodominant CD8+ T cell response to the immediate-early 2 (IE-2)-derived epitope NEGVKAAW (NW8) restricted by HLA-B^*44:03, a highly prevalent response in African populations, which in some subjects represents >10% of the circulating CD8+ T cells. Using pMHC multimer staining and sorting of NW8-specific T cells, the TCR repertoire raised against NW8 was characterised here using high-throughput sequencing in 20 HLA-B^*44

SUBMITTER: Attaf M 

PROVIDER: S-EPMC6246681 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature