Proteomic Dissection of the Cellulolytic Machineries Used by Soil-Dwelling Bacteroidetes.

ABSTRACT: Bacteria of the phylum Bacteroidetes are regarded as highly efficient carbohydrate metabolizers, but most species are limited to (semi)soluble glycans. The soil Bacteroidetes species Cytophaga hutchinsonii and Sporocytophaga myxococcoides have long been known as efficient cellulose metabolizers, but neither species conforms to known cellulolytic mechanisms. Both species require contact with their substrate but do not encode cellulosomal systems of cell surface-attached enzyme complexes or the polysaccharide utilization loci found in many other Bacteroidetes species. Here, we have fractionated the cellular compartments of each species from cultures growing on crystalline cellulose and pectin, respectively, and analyzed them using label-free quantitative proteomics as we

SUBMITTER: Taillefer M 

PROVIDER: S-EPMC6247017 | biostudies-literature | 2018 Nov-Dec

REPOSITORIES: biostudies-literature