The Capsule Regulatory Network of Klebsiella pneumoniae Defined by density-TraDISort.

ABSTRACT: Klebsiella pneumoniae infections affect infants and the immunocompromised, and the recent emergence of hypervirulent and multidrug-resistant K. pneumoniae lineages is a critical health care concern. Hypervirulence in K. pneumoniae is mediated by several factors, including the overproduction of extracellular capsule. However, the full details of how K. pneumoniae capsule biosynthesis is achieved or regulated are not known. We have developed a robust and sensitive procedure to identify genes influencing capsule production, density-TraDISort, which combines density gradient centrifugation with transposon insertion sequencing. We have used this method to explore capsule regulation in two clinically relevant Klebsiella strains, K. pneumoniae NTUH-K2044

SUBMITTER: Dorman MJ 

PROVIDER: S-EPMC6247091 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature