A 21.6 kDa tegumental protein of Clonorchis sinensis induces a Th1/Th2 mixed immune response in mice.

ABSTRACT:

Introduction

Clonorchis sinensis is a major parasite affecting the Korea population. Despite the high infection rate and pathogenicity, very few studies have been conducted to investigate the immune responses against the proteins of C. sinensis.

Methods

In this study, in vitro immune response induced by a recombinant 21.6 kDa tegumental protein derived from C. sinensis (rCsTegu21.6) was confirmed in murine dendritic cells and T cells. For the in vivo analysis, each mouse was immunized three times. Total serum IgG and T cell cytokine production were determined by ELISA, while T cell proliferation was detected by a WST (Water-Soluble Tetrazolium salt)-1 assay.

Results

In vitro tests indicated that rCsTegu21.6 treatment increased the expression of surface molecules, such

SUBMITTER: Chung E 

PROVIDER: S-EPMC6247233 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature