No difference in human mast cells derived from peanut allergic versus non-allergic subjects.

ABSTRACT:

Introduction

Mast cells are the primary effector cells of allergy. This study aimed at characterizing human peripheral blood-derived mast cells (PBdMC) from peanut allergic and non-allergic subjects by investigating whether the molecular and stimulus-response profile of PBdMC discriminate between peanut allergic and healthy individuals.

Methods

PBdMC were generated from eight peanut allergic and 10 non-allergic subjects. The molecular profile (cell surface receptor expression) was assessed using flow cytometry. The stimulus-response profile (histamine release induced by secretagogues, secretion of cytokines/chemokines and changes in miRNA expression following anti-IgE activation) was carried out with histamine release test, luminex multiplex assay and miRNA arrays.

Result

SUBMITTER: Larsen LF 

PROVIDER: S-EPMC6247235 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature