Data on spectrum-based fluorescence resonance energy transfer measurement of E. coli multidrug transporter AcrB.

ABSTRACT: This paper presented the dataset of correction parameters used in the determination of the energy transfer efficiencies from the spectrum-based fluorescence resonance energy transfer (FRET) measurement in a trimeric membrane protein AcrB. The cyan fluorescent protein (CFP) and yellow fluorescent protein (YPet) were used as the donor and acceptor, respectively. Two AcrB fusion proteins were constructed, AcrB-CFP and AcrB-YPet. The proteins were co-expressed in Escherichia coli cells, and energy transfer efficiency were determined in live cells. To obtain reliable energy transfer data, a complete set of correction parameters need to be first determined to accommodate for factors such as background fluorescence and spectra overlap. This paper described the methodology and determination

SUBMITTER: Cai Y 

PROVIDER: S-EPMC6247410 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature