Identification and characterization of a novel infectious bursal disease virus from outbreaks in Maharashtra Province of India.

ABSTRACT:

Aim

The study was undertaken to isolate infectious bursal disease virus (IBDV) from clinical cases in broiler and cockerel flocks of Maharashtra state, India, and its molecular epidemiological investigation.

Materials and methods

The morbid bursal tissues were collected from flocks suspected for IBD. The samples were subjected for virus adaptation in primary chicken embryo fibroblast (CEF) cells followed by confirmation by reverse transcription polymerase chain reaction (RT-PCR) for partial VP₂ sequence and phylogenetic analysis.

Results

The isolation of IBDV from field samples took seven blind passages for adaptation in CEF. The cytopathic effects included rounding, aggregation, vacuolation, and detachment of the cells. The RT-PCR showed amplification of 6

SUBMITTER: Awandkar SP 

PROVIDER: S-EPMC6247889 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature