12-HETE facilitates cell survival by activating the integrin-linked kinase/NF-κB pathway in ovarian cancer.

ABSTRACT:

Background

The dysfunction of cell apoptosis is an important event in the progression of cancer, and the growth of cancer cells is negatively regulated by cell apoptosis. In different types of cancers, inhibition of cellular apoptosis is often observed in the cancerous tissue, and increased resistance to apoptosis is a hallmark of cancer. Although previous studies have shown that 12-lipoxygenase (12-LOX)/12-hydroxyeicosatetraenoic acid (12-HETE) is activated and upregulated in different types of cancers, the consequences of 12-LOX/12-HETE upregulation and its precise roles in the survival of ovarian carcinoma cells are still unknown.

Methods

MTT assays, caspase activity assays, lactate dehydrogenase (LDH) assays, and Western blot analysis were the methods used in this study.

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6248369 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature