The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis.

ABSTRACT: Telomerase RNA (TR) provides the template for DNA repeat synthesis at telomeres and is essential for genome stability in continuously dividing cells. We mapped the RNA interactome of human TR (hTR) and identified a set of non-coding and coding hTR-interacting RNAs, including the histone 1C mRNA (HIST1H1C). Disruption of the hTR-HIST1H1C RNA association resulted in markedly increased telomere elongation without affecting telomerase enzymatic activity. Conversely, over-expression of HIST1H1C led to telomere attrition. By using a combination of mutations to disentangle the effects of histone 1 RNA synthesis, protein expression, and hTR interaction, we show that HIST1H1C RNA negatively regulates telomere length independently of its protein coding potential. Taken to

SUBMITTER: Ivanyi-Nagy R 

PROVIDER: S-EPMC6249008 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature