Generation of uniform-sized multicellular tumor spheroids using hydrogel microwells for advanced drug screening.

ABSTRACT: Even though in vitro co-culture tumor spheroid model plays an important role in screening drug candidates, its wide applications are currently limited due to the lack of reliable and high throughput methods for generating well-defined and 3D complex co-culture structures. Herein, we report the development of a hydrogel microwell array to generate uniform-sized multicellular tumor spheroids. Our developed multicellular tumor spheroids are structurally well-defined, robust and can be easily transferred into the widely used 2D culture substrates while maintaining our designed multicellular 3D-sphere structures. Moreover, to develop effective anti-cancer therapeutics we integrated our recently developed gold-graphene hybrid nanomaterial (Au@GO)-based photothermal cancer therapy into a series o

SUBMITTER: Lee JM 

PROVIDER: S-EPMC6249215 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature