DHAV-1 2A1 Peptide - A Newly Discovered Co-expression Tool That Mediates the Ribosomal "Skipping" Function.

ABSTRACT: Duck hepatitis A virus 1 (DHAV-1) belongs to the genus Avihepatovirus in the family Picornaviridae. Little research has been carried out on the non-structural proteins of this virus. This study reports that 2A1 protein, the first non-structural protein on the DHAV-1 genome, has a ribosomal "skipping" function mediated by a "-GxExNPGP-" motif. In addition, we prove that when the sequence is extended 10aa to VP1 from the N-terminal of 2A1, the ribosome "skips" completely. However, as the N-terminus of 2A is shortened, the efficiency of ribosomal "skipping" reduces. When 2A1 is shortened to 10aa, it does not function. In addition, we demonstrate that N¹⁸, P¹⁹ G²⁰, and P²¹ have vital roles in this function. We find that the expression o

SUBMITTER: Yang X 

PROVIDER: S-EPMC6249498 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature