Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae.

ABSTRACT: In the budding yeast Saccharomyces cerevisiae, transcription factors (TFs) regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events. Experimental evidence coupled with quantitative models suggests that a network of interconnected TFs is capable of regulating periodic genes over the cell cycle. Importantly, these dynamical models were built on transcriptomics data and assumed that TF protein levels and activity are directly correlated with mRNA abundance. To ask whether TF transcripts match protein expression levels as cells progress through the cell cycle, we applied a multiplexed targeted mass spectrometry approach (parallel reaction monitoring) to synchronized populations of cells. We found that prote

SUBMITTER: Kelliher CM 

PROVIDER: S-EPMC6249835 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature