Stem cell library screen identified ruxolitinib as regulator of osteoblastic differentiation of human skeletal stem cells.

ABSTRACT:

Background

Better understanding of the signaling pathways that regulate human bone marrow stromal stem cell (hBMSC) differentiation into bone-forming osteoblasts is crucial for their clinical use in regenerative medicine. Chemical biology approaches using small molecules targeting specific signaling pathways are increasingly employed to manipulate stem cell differentiation fate.

Methods

We employed alkaline phosphatase activity and staining assays to assess osteoblast differentiation and Alizarin R staining to assess mineralized matrix formation of cultured hBMSCs. Changes in gene expression were assessed using an Agilent microarray platform, and data normalization and bioinformatics were performed using GeneSpring software. For in vivo ectopic bone formation experiments, hM

SUBMITTER: AlMuraikhi N 

PROVIDER: S-EPMC6249887 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature