Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.

ABSTRACT: High-content real-time imaging of hormone secretion in tissues or cell populations is a challenging task, which is unlikely to be resolved directly, despite immense translational value. We approach this problem indirectly, using compensatory endocytosis, a process that closely follows exocytosis in the cell, as a surrogate read-out for secretion. The tissue is immobilized in an open-air perifusion chamber and imaged using a two-photon microscope. A fluorescent polar tracer, perifused through the experimental circuit, gets trapped into the cells via endocytosis, and is quantified using a feature-detection algorithm. The signal of the tracer that accumulates into the endocytotic system reliably reflects stimulated exocytosis, which is demonstrated via co-imaging of the latter using existing

SUBMITTER: Tarasov AI 

PROVIDER: S-EPMC6250124 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature