Renal Klotho is Reduced in Septic Patients and Pretreatment With Recombinant Klotho Attenuates Organ Injury in Lipopolysaccharide-Challenged Mice.

ABSTRACT:

Objectives

To determine the applicability of recombinant Klotho to prevent inflammation and organ injury in sepsis in man and mice.

Design

Prospective, clinical laboratory study using "warm" human postmortem sepsis-acute kidney injury biopsies. Laboratory study using a mouse model of endotoxemia.

Setting

Research laboratory at a university teaching hospital.

Subjects

Adult patients who died of sepsis in the ICU and control patients undergoing total nephrectomy secondary to renal cancer; male C57BL/6 and Klotho haploinsufficient mice.

Interventions

Lipopolysaccharide (0.05 mg/kg) injection and kill after 4, 8, and 24 hours. Mice received recombinant Klotho (0.05 mg/kg) 30 minutes prior to lipopolysaccharide (1 mg/kg) injection. Mice treated with saline we

SUBMITTER: Jou-Valencia D 

PROVIDER: S-EPMC6250245 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature