Keratinocyte Migration in a Three-Dimensional In Vitro Wound Healing Model Co-Cultured with Fibroblasts.

ABSTRACT:

Background

Because three-dimensional (3D) models more closely mimic native tissues, one of the goals of 3D in vitro tissue models is to aid in the development and toxicity screening of new drug therapies. In this study, a 3D skin wound healing model comprising of a collagen type I construct with fibrin-filled defects was developed.

Methods

Optical imaging was used to measure keratinocyte migration in the presence of fibroblasts over 7 days onto the fibrin-filled defects. Additionally, cell viability and growth of fibroblasts and keratinocytes was measured using the alamarBlue^® assay and changes in the mechanical stiffness of the 3D construct was monitored using compressive indentation testing.

Results

Keratinocyte migration rate was significantly inc

SUBMITTER: Iyer K 

PROVIDER: S-EPMC6250652 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature