The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients.

ABSTRACT: Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-naïve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower t

SUBMITTER: Moen AEF 

PROVIDER: S-EPMC6250705 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature