Development of a multi-locus CRISPR gene drive system in budding yeast.

ABSTRACT: The discovery of CRISPR/Cas gene editing has allowed for major advances in many biomedical disciplines and basic research. One arrangement of this biotechnology, a nuclease-based gene drive, can rapidly deliver a genetic element through a given population and studies in fungi and metazoans have demonstrated the success of such a system. This methodology has the potential to control biological populations and contribute to eradication of insect-borne diseases, agricultural pests, and invasive species. However, there remain challenges in the design, optimization, and implementation of gene drives including concerns regarding biosafety, containment, and control/inhibition. Given the numerous gene drive arrangements possible, there is a growing need for more advanced designs. In this study, we

SUBMITTER: Yan Y 

PROVIDER: S-EPMC6250742 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature