Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.

ABSTRACT:

Background

Cone and rod photoreceptors are two of the primary cell types affected in human retinal disease. Potential strategies to combat these diseases are the use of gene therapy to rescue compromised photoreceptors or to generate new functional photoreceptors to replace those lost in the diseased retina. Cis-regulatory elements specific to cones, rods, or both types of photoreceptors are critical components of successful implementation of these two strategies. The purpose of this study was to identify and characterize the cell type specificity and activity of cis-regulatory elements active in developing photoreceptors.

Methods

Cis-regulatory elements were introduced into the developing chicken and mouse retina by electroporation. Characterization of reporter activity in

SUBMITTER: Jean-Charles N 

PROVIDER: S-EPMC6251108 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature