High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.).

ABSTRACT:

Background

In African tsetse flies Glossina, spp. detection of bacterial symbionts such as Wolbachia is challenging since their prevalence and distribution are patchy, and natural symbiont titers can range at levels far below detection limit of standard molecular techniques. Reliable estimation of symbiont infection frequency, especially with regard to interrelations between symbionts and their potential impact on host biology, is of pivotal interest in the context of future applications for the control and eradication of Glossina-vectored African trypanosomosis. The presence or absence of symbionts is routinely screened with endpoint polymerase chain reaction (PCR), which has numerous advantages, but reaches its limits, when detecting infections at natural low titer. To not only d

SUBMITTER: Schneider DI 

PROVIDER: S-EPMC6251158 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature