Comparative analysis of inosine-substituted duplex DNA by circular dichroism and X-ray crystallography.

ABSTRACT: Leveraging structural biology tools, we report the results of experiments seeking to determine if the different mechanical properties of DNA polymers with base analog substitutions can be attributed, at least in part, to induced changes from classical B-form DNA. The underlying hypothesis is that different inherent bending and twisting flexibilities may characterize non-canonical B-DNA, so that it is inappropriate to interpret mechanical changes caused by base analog substitution as resulting simply from 'electrostatic' or 'base stacking' influences without considering the larger context of altered helical geometry. Circular dichroism spectra of inosine-substituted oligonucleotides and longer base-substituted DNAs in solution indicated non-canonical helical conformations, with the degree o

SUBMITTER: Peters JP 

PROVIDER: S-EPMC6251417 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature