DNA recognition patterns of the multi-zinc-finger protein CTCF: a mutagenesis study.

ABSTRACT: CCCTC-binding factor (CTCF) is a zinc-finger protein, serving an important part in the genome architecture as well as some biochemical processes. Over 70,000 CTCF binding DNA sites have been detected genome-wide, and most anchors of chromatin loops are demarcated with the CTCF binding. Various protein or RNA molecules interact with DNA-bound CTCF to conduct different biological functions, and potentially the interfaces between CTCF and its cofactors can be targets for drug development. Here we identify the effective region of CTCF in DNA recognition, which defines the exposed CTCF surface feature for the interaction of cofactors. While the zinc-finger region contributes the most in DNA association, its binding affinity varies based on different DNA sequences. To investigate the effectivene

SUBMITTER: Guo J 

PROVIDER: S-EPMC6251859 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature