AutoFoci, an automated high-throughput foci detection approach for analyzing low-dose DNA double-strand break repair.

ABSTRACT: Double-strand breaks (DSBs) are the most lethal DNA damages induced by ionising radiation (IR) and their efficient repair is crucial to limit genomic instability. The cellular DSB response after low IR doses is of particular interest but its examination requires the analysis of high cell numbers. Here, we present an automated DSB quantification method based on the analysis of γH2AX and 53BP1 foci as markers for DSBs. We establish a combination of object properties, combined in the object evaluation parameter (OEP), which correlates with manual object classification. Strikingly, OEP histograms show a bi-modal distribution with two maxima and a minimum in between, which correlates with the manually determined transition between background signals and foci. We used algorithms to detect the mi

SUBMITTER: Lengert N 

PROVIDER: S-EPMC6251879 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature