Inclusion Body Expression and Refolding of Recombinant Bone Morphogenetic Protein-2.

ABSTRACT:

Background

Bone Morphogenetic Protein-2 (BMP-2) is a cysteine rich growth factor expressed in homodimeric form and has a pivotal role in osteochondral development and fracture healing. Recent studies have benefited more from recombinant BMP-2 in osteochondral tissue engineering. Cost-effective and easy production at large scale makes Escherichia coli (E. coli) the first choice for recombinant protein expression programs. However, inclusion body aggregation and refolding process limits production and purification of recombinant BMP-2 in bacterial systems.

Methods

BMP-2 encoded gene was optimized for expression in bacterial expression system and synthesized with proper restriction sites. The optimized sequence was then cloned in a pET28a expression vector and exp

SUBMITTER: Nasrabadi D 

PROVIDER: S-EPMC6252035 | biostudies-literature | 2018 Oct-Dec

REPOSITORIES: biostudies-literature