Adjuvant selection regulates gut migration and phenotypic diversity of antigen-specific CD4⁺ T cells following parenteral immunization.

ABSTRACT: Infectious diarrheal diseases are the second leading cause of death in children under 5 years, making vaccines against these diseases a high priority. It is known that certain vaccine adjuvants, chiefly bacterial ADP-ribosylating enterotoxins, can induce mucosal antibodies when delivered parenterally. Based on this, we reasoned vaccine-specific mucosal cellular immunity could be induced via parenteral immunization with these adjuvants. Here, we show that, in contrast to the Toll-like receptor-9 agonist CpG, intradermal immunization with non-toxic double-mutant heat-labile toxin (dmLT) from enterotoxigenic Escherichia coli drove endogenous, antigen-specific CD4⁺ T cells to expand and upregulate the gut-homing integrin α4β7. This was followed by T-cell migration into gut-draining

SUBMITTER: Frederick DR 

PROVIDER: S-EPMC6252260 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature