Morpho-histological development of the somatic embryos of Typha domingensis.

ABSTRACT:

Background

Sustainable methods of propagation of Typha domingensis through somatic embryogenesis can help mitigate its current condition of ecological marginalization and overexploitation. This study examined whether differentiation up to coleoptilar embryos could be obtained in an embryogenic line proliferated with light and high auxin concentration.

Methods

Murashige and Skoog medium at half ionic strength and containing 3% sucrose and 0.1% ascorbic acid was used for the three embryogenic phases. Induction started with aseptic 9-day-old germinated seeds cultured in 0.5 mg L^-1 2,4-dichlorophenoxyacetic (2,4-D). Proliferation of the embryogenic callus was evaluated at 2,4-D concentrations ranging from 0 to 2 mg L^-1 in cultures maintained in the d

SUBMITTER: Hernandez-Piedra G 

PROVIDER: S-EPMC6254243 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature