Depletion of plasma membrane-associated phosphoinositides mimics inhibition of TRPM7 channels by cytosolic Mg²⁺, spermine, and pH.

ABSTRACT: Transient receptor potential cation channel subfamily M member 7 (TRPM7) is an ion channel/protein kinase belonging to the TRP melastatin and eEF2 kinase families. Under physiological conditions, most native TRPM7 channels are inhibited by cytoplasmic Mg²⁺, protons, and polyamines. Currents through these channels (I_TRPM7) are robustly potentiated when the cell interior is exchanged with low Mg²⁺-containing buffers. I_TRPM7 is also potentiated by phosphatidyl inositol bisphosphate (PI(4,5)P₂) and suppressed by its hydrolysis. Here we characterized internal Mg²⁺- and pH-mediated inhibition of TRPM7 channels in HEK293 cells overexpressing WT voltage-sensing phospholipid phosphatase (VSP) or its catalytically inactive variant VSP

SUBMITTER: Zhelay T 

PROVIDER: S-EPMC6254349 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature